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| Furian,TQ; Borges,KA; Pilatti,RM; Almeida,C; Nascimento,VP do; Salle,CTP; Moraes,HL de S. |
| The ability of Pasteurella multocida to invade and multiply in its host is enhanced by the presence of the capsule, one of the most important virulence factors for this bacterium. Capsular typing methods are often used in epidemiological and pathogenesis studies of this agent. Five different serogroups have been identified based on serological typing. However, such tests are laborious, and agglutination of homologous antiserum may fail. The aim of this study was to develop a multiplex PCR protocol for the identification of the hyaD-hyaC and dcbF genes specific to serogroups A and D, respectively, and to compare these results with those of phenotypic tests for 54 strains isolated from fowl cholera cases in southern Brazil. The kappa coefficient and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular diagnosis; Non-serologic tests; Pasteurellosis; Serogroup. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000200004 |
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| Rauber,RH; Perlin,VJ; Fin,CD; Mallmann,AL; Miranda,DP; Giacomini,LZ; Nascimento,VP do. |
| This study was conducted to determine the interference of Salmonella typhimurium lipopolysaccharide (sLPS) on the performance, biological parameters, and histological evaluations of 198 one-day-old male broiler chickens divided into three treatments according to sLPS dose (0, 250, or 500 µg/application/bird) that was applied to the birds every other day, from 15 to 27 days of age. At the end of the experiment (28 days), significant effects were observed on body weight (R= -0.17 and P=0.05), total cholesterol serum levels(R=0.43 and p<0.01), phosphorus (R=0.53 and P<0.01), uric acid (R= -0.38 and P<0.01), C-reactive protein (R=0.68 and p<0.01), serum activity of aspartate aminotransferase (R=0.39 and p<0.01) and alkaline phosphatase (R= -0.39... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Poultry; Clinical biochemistry; Endotoxin; Body weight. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000100011 |
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| Colla,FL; Mion,L; Parizotto,L; Rodrigues,LB; Pilotto,F; Dickel,EL; Nascimento,VP do; Santos,LR dos. |
| Salmonella is traditionally identified by conventional microbiological tests, but the enumeration of this bacterium is not used on a routine basis. Methods such as the most probable number (MPN), which utilize an array of multiple tubes, are time-consuming and expensive, whereas miniaturized most probable number (mMPN) methods, which use microplates, can be adapted for the enumeration of bacteria, saving up time and materials. The aim of the present paper is to assess two mMPN methods for the enumeration of Salmonella sp in artificially-contaminated chicken meat samples. Microplates containing 24 wells (method A) and 96 wells (method B), both with peptone water as pre-enrichment medium and modified semi-solid Rappaport-Vassiliadis (MSRV) as selective... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken meat; Most probable number; Salmonella. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000100006 |
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